Journal: Open Life Sciences

Article Title: Effects and potential mechanisms of luteolin on cisplatin mediated inhibition of cervical adenocarcinoma

doi: 10.1515/biol-2025-1274

Figure Lengend Snippet: Luteolin inhibits the proliferation and colonization capacity of HeLa cells. HeLa cells were treated with different concentrations of LU (0, 0.1, 0.5, 5, 10, 20, 40 µM 0 μM) and CDDP (0, 0.5, 2.5 μg/mL) for 24 h and 48 h. A and B. HeLa cell proliferation induced by different concentrations of LU and CDDP for 24 h and 48 h was detected by CCK8 assay. C and D. Siha cell proliferation induced by different concentrations of LU and CDDP for 24 h and 48 h was detected by CCK8 assay. E and F. The number and morphology of hela and siha cells were observed with microscope. G and H. HeLa cell proliferation was detected by colony formation assay and statistical analysis. * P < 0.05, ** P < 0.01 compared with the control group.

Article Snippet: The human cervical cancer HeLa and Siha cell lines were obtained from Procell Life Science & Technology Co., Ltd.

Techniques: CCK-8 Assay, Microscopy, Colony Assay, Control

Journal: Cancers

Article Title: Differential Protumoral Mechanisms Induced by CAFs in Cervical Cancer Cells Occur Independently of 17β-Estradiol Stimulation

doi: 10.3390/cancers18101509

Figure Lengend Snippet: CAFs decrease the mitochondrial metabolism and enhance the ROS production in SiHa cells. Mitochondrial metabolism was assessed by MTT assay, and ROS by H2DCFDA. SiHa and HeLa cells were cultured with LG or HG CAFs’ supernatant prior to metabolism or ROS measurement. ( A ) Mitochondrial metabolism of SiHa and HeLa cells stimulated with LG and HG CAFs, and metabolic activity was normalized against the control. ( B ) Production of ROS by SiHa and HeLa cells stimulated with LG and HG CAFs. Statistical analysis was first performed with a normality test using the Shapiro–Wilk test and later with Kruskal–Wallis or one-way ANOVA. Data is presented as mean ± SD. * p < 0.05, ** p < 0.01, **** p < 0.0001. LG CAF: Cancer-associated fibroblasts from low-grade lesion, HG CAF: Cancer-associated fibroblasts from high-grade lesion, Basal: Cells cultured with DMEM, CAF basal: Cells cultured with CAF’s supernatant without E2 stimulation, CAF E2: Cells cultured with CAF’s supernatant with previous stimulation with E2.

Article Snippet: CC cell lines SiHa and HeLa were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: MTT Assay, Cell Culture, Activity Assay, Control

Journal: Cancers

Article Title: Differential Protumoral Mechanisms Induced by CAFs in Cervical Cancer Cells Occur Independently of 17β-Estradiol Stimulation

doi: 10.3390/cancers18101509

Figure Lengend Snippet: CAFs promote cell migration in HeLa cells. Migration was assessed using a wound-healing assay and quantified as the percentage of wound closure. ( A ) Migration of SiHa cultured with LG and HG cervical cancer lesion CAFs’ supernatant. ( B ) Migration of HeLa cultured with LG and HG cervical cancer lesion CAFs’ supernatant. In photographs, the yellow color is used to highlight the measured % of area in the assay. Statistical analysis was performed first with a normality test using the Shapiro–Wilk test and later using Kruskal–Wallis for panel A and one-way ANOVA for panel B. Data are presented as mean ± SD. * p < 0.05, *** p < 0.001, **** p < 0.0001. LG CAF: Cancer-associated fibroblasts from low-grade lesion, HG CAF: Cancer-associated fibroblasts from high-grade lesion, Basal: Cells cultured with DMEM, CAF: Cells cultured with CAF’s supernatant without E2 stimulation, CAF E2: Cells cultured with CAF’s supernatant with previous stimulation with E2.

Article Snippet: CC cell lines SiHa and HeLa were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Migration, Wound Healing Assay, Cell Culture

Journal: Cancers

Article Title: Differential Protumoral Mechanisms Induced by CAFs in Cervical Cancer Cells Occur Independently of 17β-Estradiol Stimulation

doi: 10.3390/cancers18101509

Figure Lengend Snippet: CAFs do not affect apoptosis in CC cell lines. Apoptosis was assessed by Annexin V staining for phosphatidylserine exposure, and cell death by PI staining, with results expressed as percentages. ( A ) Apoptosis and death of SiHa cultured with low- and high-grade cervical cancer lesion CAFs’ supernatant. ( B ) apoptosis and death of HeLa cultured with low- and high-grade cervical cancer lesion CAFs’ supernatant. Statistical analysis was first performed with a normality test using the Shapiro–Wilk test, followed by Kruskal–Wallis or one-way ANOVA. Data is presented as mean ± SD. ns: Not significant. C-: Negative control, only cultured with DMEM, LG: Cancer-associated fibroblasts from low-grade lesion, LGE2: Cancer-associated fibroblasts from low-grade lesion with previous E2 stimulation, CIS: Cisplatin 80 μg/mL, LG CIS: Cancer-associated fibroblasts from low-grade lesion + cisplatin 80 μg/mL, LGE2 CIS: Cancer-associated fibroblasts from low-grade lesion with previous E2 stimulation + cisplatin 80 μg/mL, HG: Cancer-associated fibroblasts from high-grade lesion, HGE2: Cancer-associated fibroblasts from high-grade lesion with previous E2 stimulation, HG CIS: Cancer-associated fibroblasts from high-grade lesion + cisplatin 80 μg/mL, HGE2 CIS: Cancer-associated fibroblasts from high-grade lesion with previous E2 stimulation + cisplatin 80 μg/mL.

Article Snippet: CC cell lines SiHa and HeLa were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Staining, Cell Culture, Negative Control

Journal: Oncology Letters

Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer

doi: 10.3892/ol.2026.15486

Figure Lengend Snippet: HMGN1 inhibition affects the proliferation of cervical squamous cells. (A) mRNA and (B) protein expression levels of HMGN1 in SiHa and HeLa cells following siRNA transfection, compared with the NC. (C) Cell Counting Kit-8 assay assessing the proliferation of SiHa and HeLa cells at 24, 48 and 72 h. (D) Cell cycle distribution of SiHa and HeLa cells after HMGN1 inhibition was analyzed by flow cytometry, including flow cytometry plots and quantitative histograms. *P<0.05, **P<0.01 and ****P<0.001. HMGN1, high-mobility group nucleosome-binding protein 1; NC, non-targeting control; si, small interfering RNA.

Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).

Techniques: Inhibition, Expressing, Transfection, Cell Counting, Flow Cytometry, Binding Assay, Control, Small Interfering RNA